MagSi-DNA Plant CLS

Extraction of Genomic DNA from Plant Cotyl, Leaf and Seed

Plant DNA markers can be used to identify desirable traits in crops, such as disease resistance, drought tolerance, and increased yield. This allows for more efficient breeding and the production of higher quality crops.

MagSi-DNA Plant CLS kit allows fast and cost-effective extraction of DNA from any plant tissue type. CLS stands for Cotyl, Leaf and Seed, which are the most commonly used sample types in Plant Breeding.

The MagSi-DNA Plant CLS kit contains all the components necessary to optimize your specific DNA extraction procedure, with regards to yield, purity, and working volumes.

The kit can be used on any open DNA extraction robot or liquid handling system. The kit has been tested with many different plant species.

Features

  • Kit is especially designed for workflow efficiency
  • Contains 2 lysis buffers for different samples in 1
    extraction procedure
  • Preparation time for 96 samples: 25 min. after lysis
  • High yields and excellent DNA purity
  • Suitable for downstream applications such as SNP genotyping by PCR or NGS
  • No phenol-chloroform extraction
  • Ready-to-use kit – Avoiding adding alcohols
  • Fast and convenient protocols that are easily adaptable to high-throughput automation

Table 1. Overview of plant species tested (not exhaustive)

AMOUNT
AMOUNT
AMOUNT
Barley
Melon
Sugar beet
Maize
Asparagus
Tomato
Rice
Cucumber
Turnip
Wheat
Lettuce
Chrysant
Rape
Onion
Wheat
Lettuce
Gerbera
Soy
Sunflower
Spinach
Petunia
Strawberry
Squash
Rose

Ordering Information

ART.NO.
DESCRIPTION
AMOUNT
MDKT00260096
MagSi-DNA Plant CLS
96 preps
MDKT00260960
MagSi-DNA Plant CLS
10 x 96 preps
MDKT00260196PF
rQ MagSi-DNA <br>Plant CLS *
96 preps
MD0200142596PF
rQ Plant CLS Binding Plate **
25 pcs

* All kit components except lysis buffers provided pre-filled in 96 DeepWell plates for PurePrep 96 / KingFisherTM Flex 96

** Pre-filled binding plate contains beads and binding buffer suited for PurePrep 96 / KingFisherTM Flex 96

Request info and get
50% discount…

Fill out the form below, and we will give you a 50% discount on your next MagSi-DNA Plant CLS kit order*
*on first order only; regardless order size; use promo-code provided after submitting; valid until Dec 22, 2023

Pre-filled Magnetic Beads and Binding Buffer: rQ Plant CLS Binding Plate

After evaluation and finding the ideal set of reagents for your DNA extraction workflow, we can offer a unique, customized, pre-filled version of the key components: the magnetic beads and the binding buffer in a microplate, the rQ Plant CLS Binding Plate.

  • Eliminate all beads handling issues
  • Save time and costs on reagent addition
  • Save costs on beads mixing equipment
  • Increase instrument capacity
  • Environmentally friendly

 

Magnetic beads and binding buffer can be supplied in microplates for PurePrep or KingFisherTM, or your specific extraction plates (on liquid handling systems).

Ask for our customized bulk options and accessory products

TECHNICAL DATA | MagSi-DNA Plant CLS

Figure 1. DNA concentrations extracted from vegetable, field crop and oil seeds, measured by UV-VIS with the NanoDropTM One. The data are presented as mean (n=3, ±1 SD).
Figure 2. DNA purity ratios for tomato, cucumber, pepper, corn, sugar beet, wheat, rape, sunflower and soybean seeds obtained by UV-VIS with the NanoDropTM One. High purity ratios are measured for all seeds. Data are presented as mean (n=3, ±1 SD).

Figure 3. PCR results from extracted vegetable, field crop and oil seed DNA with primers targeting the tRNA-leucine gene. The data are presented as mean (n=3, ±1 SD).

PRODUCT RESOURCES | MagSi-DNA Plant CLS

Catalog

All our magnetic beads, kits and separators in one overview.

Products for magnetic sample preparation

Leaflets

Commercial leaflets of our magnetic separation products.

Manuals

Manuals on how to use our magnetic separation solutions.