MagSi-DNA FFPE
DNA from FFPE preserved tissue
FFPE stands for “Formalin-Fixed Paraffin-Embedded,” and it refers to a common method of preserving and storing biological tissue samples for histological analysis and molecular studies. FFPE tissue is widely used in pathology, research, and clinical diagnostics.
MagSi-DNA FFPE
This kit is intended for manual and automated isolation of genomic DNA from mammalian FFPE tissue or cell samples. Processing time for the preparation of 96 samples is about 40 minutes plus an additional paraffin removal step and lysis incubation.
Safety
The kit does not require phenol/chloroform extraction or alcohol precipitation and eliminates the need for repeated centrifugation, vacuum filtration or column separation. It allows safe handling of potentially infectious samples, and is designed to avoid sample-to-sample cross-contaminations. The obtained DNA can be used directly for downstream applications such as PCR, NGS, or any kind of enzymatic reaction.
Features
- Short and easy protocols, 96 samples in 40 minutes
- Multiple options for deparaffinization
- Eliminates need for repeated centrifugation, vacuum filtration or column separations
- Consistently high yield and purity of genomic DNA
- Suitable for many downstream applications
- Depending on the sample materials RNA may be co-purified. If required RNase treatment has to be integrated in the purification protocol. RNase is not included in the kit (available separately)
- Easy to automate
- PurePrep/ KingFisherTM/ BioSprint 96/ MagMaxTM protocols and consumables available
- Compatible with general liquid handling robots (e.g. Hamilton®, TECAN®)
- Magnetic separators for microtubes and microplates separately available for convenient manual or automated DNA extractions
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ART.NO.
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DESCRIPTION
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AMOUNT
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MDKT00240096
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MagSi-DNA FFPE
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96 preps
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MDKT00240960
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MagSi-DNA FFPE
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10 x 96 preps
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TECHNICAL DATA | MagSi-DNA FFPE
Figure 1. DNA concentrations (NanoDrop) obtained from different FFPE samples (liver, lung, cells) after extraction vs competitor.
Figure 2. DNA concentrations (NanoDrop) obtained from 1 to 4 scrolls (20 µm) of FFPE embedded cells. Corresponding DNA purity ratios A260/A280 range from 1.74 to 1.89. Sample deparaffinization was achieved using the melting procedure by heat incubation without xylene, mineral oil or other organic solvents.
Figure 3. Ct values obtained by qPCR (beta-actin) on purified lung, liver and cell samples versus competitor. Purified samples were used undiluted in the qPCR reaction. No PCR inhibition was observed.
