MagSi-NA Pathogens

Total nucleic acid extraction for pathogen detection (suited for Covid-19)

The MagSi-NA Pathogens kit allows cost-effective extraction of DNA and RNA from a variety of sample materials like serum, plasma, oropharyngeal swab, nasopharyngeal swab, or any other respiratory samples. Purified total nucleic acids can be used for qPCR based or any other enzymatic pathogen detection method. The ready-to-use reagents and simple protocol are convenient in use and easy to automate. The included MagSi-PA VII magnetic beads are optimized for fast separation even from viscous sample lysates.

Features

  • Short protocols, complete processing at room temperature possible
  • Consistently high yield of total nucleic acids
  • Very strong magnetic beads enable fast magnetic separation even from viscous sample lysates
  • Suitable for many enzymatic down-stream applications including qPCR, qRT-PCR isothermal amplification
  • Preparation time for 96 samples: <30 min
  • Easy to automate with PurePrep systems
  • Collect your samples pain-free with the SafeQ Saliva Collection Kit
  • Save costs while maintaining sensitivity: Magnetic Sample Pooling
ART.NO.
DESCRIPTION
AMOUNT
MDKT00210960
MagSi-NA Pathogens
10 x 96 preps
MDKT0021025K
MagSi-NA Pathogens
25K preps
MDKT0021005K
MagSi-NA Pathogens
5K preps
MDKT00210096
MagSi-NA Pathogens
96 preps
MDKT0021BULK
MagSi-NA Pathogens
Customized BULK
MDKT0021P06K
MagSi-NA Pathogens MSP
up to 6000 samples*

Accessory materials to be offered with MagSi kits for DNA extraction

All DNA extraction kits include a final drying step to remove traces of ethanol before DNA elution.
Wash Buffer III eliminates this step, resulting in faster protocols and DNA with higher purity.

ART.NO.
DESCRIPTION
AMOUNT
MDBU00111000
Wash Buffer III
1000 mL
MDBU00115000
Wash Buffer III
5000 mL

TECHNICAL DATA | MagSi-NA Pathogens

Figure 1: Variable amounts of MS phage RNA were spiked to human serum samples. MS2 RNA was detected using a qRT-PCR assay. High recovery rates were obtained with reference to the spiked RNA (Input) and in comparison to a competitive kit (Product G).

 

Figure 2: Variable amounts of lambda DNA were spiked to human serum samples. Lambda DNA was detected using a qPCR assay. High recovery rates were obtained with reference to the spiked DNA (input) and in comparison to a competitive kit (Product G).

 

Figure 3: Variable amounts of SARS-CoV-2 positive saliva samples were diluted in SARS-CoV-2 negative saliva. RNA target sequences for SARS-CoV-2 (S- and N2-gene) were detected using a qRT-PCR assay. Homogeneous amplification of the endogenous control gene (IC, beta-Actin) was obtained in all samples, indicating absence of PCR inhibition.

PRODUCT RESOURCES | MagSi-NA Pathogens

Catalog

All our magnetic beads, kits and separators in one overview.

Videos

Demonstration movies of our kits and automation solutions

Manuals

Manuals on how to use our magnetic separation solutions.