MagSi-DNA Tissue & Cells

Isolation of genomic DNA from mammalian tissue and eukaryotic cells

The MagSi-DNA Tissue & Cells kit is intended for manual and automated isolation of genomic DNA from mammalian tissue samples and eukaryotic cells. Processing time for the preparation of 96 samples is about 40 minutes plus an additional pre-lysis incubation of 15 min (for cells), 1-3 h or overnight for tissue samples.

The kit does not require phenol/chloroform extraction or alcohol precipitation and eliminates the need for repeated centrifugation, vacuum filtration or column separation. It allows safe handling of potentially infectious samples, and is designed to avoid sample-to-sample cross-contaminations. The obtained DNA can be used directly for downstream applications such as PCR, or any kind of enzymatic reaction.

Features

  • Short and easy protocols, 96 samples in 40 minutes
  • Avoid dangerous substances like phenol/chloroform
  • Eliminates need for repeated centrifugation, vacuum or column separations
  • Consistently high yield and purity of genomic DNA
  • Excellent integrity DIN > 8.1
  • Suitable for many downstream applications


Easy to automate

  • Minimal accessory requirements
  • PurePrep / KingFisherTM / Biosprint 96 / MagMaxTM protocols and consumables available
  • Compatible with general liquid handling robots (e.g. Hamilton®, TECAN®)
  • Magnetic separators for microtubes and microplates separately available
ART.NO.
DESCRIPTION
AMOUNT
MDKT00180096
MagSi-DNA Tissue & Cells
96 preps
MDKT00180960
MagSi-DNA Tissue & Cells
10 x 96 preps
MDKT0018BULK
MagSi-DNA Tissue & Cells
BULK

Accessory materials to be offered with MagSi kits for DNA extraction

All DNA extraction kits include a final drying step to remove traces of ethanol before DNA elution.
Wash Buffer III eliminates this step, resulting in faster protocols and DNA with higher purity.

ART.NO.
DESCRIPTION
AMOUNT
MD73041
Wash Buffer III
1000 mL
MD74041
Wash Buffer III
5000 mL

TECHNICAL DATA | MagSi-DNA Tissue & Cells

Figure 1: DNA concentrations obtained from different mouse tissues (liver, brain, lung, tail) after extraction vs competitor.

Figure 2: Ct values obtained by qPCR (GAPDH) on different mouse tissues after extraction vs competitor.

Figure 3: Automated electrophoresis by Agilent TapeStation 4150 showing high integrity of purified DNA from frozen mouse liver tissue. All DNA Integrity Numbers (DIN) are >8.1 (not shown).

PRODUCT RESOURCES | MagSi-DNA Tissue & Cells

Catalog

All our magnetic beads, kits and separators in one overview.

Manuals

Manuals on how to use our magnetic separation solutions.

Leaflets

Commercial leaflets of our magnetic separation products.

Technical Notes

Technical application of our magnetic beads and kit’s solutions.