While saving money on extractions and PCR reactions, classic sample pooling will still dilute your samples and therefore cause less sensitive and false negative results. Our solution, Magnetic Sample Pooling (MSP) does not only save reagents, but maintains your test sensitivity as it pools in a sequential and non-dilutive manner.
Up to 6 times 96 samples are added to the wells of up to 6 deepwell plates. First, all samples are lysed under denaturing conditions by addition of lysis buffer including proteinase K and Poly-A-RNA. Magnetic MSP beads and binding buffer are then added to every sample.
During the isolation procedure the magnetic MSP beads will bind the nucleic acids from all (up to 6) samples by transferring them in a serial manner to the next sample plate, until all (up to 6) samples have been incubated with magnetic MSP beads.
After this, the magnetic MSP beads are washed in 3 deepwell plates containing alcoholic buffers. Finally, the nucleic acids are collected from the sample plates and released into an elution plate using a low-salt elution buffer. They can then be directly used for downstream applications.
Diagram: How Magnetic Sample Pooling (MSP) works
MagSi-NA Pathogens MSP
up to 6000 samples*
* in case of 6:1 pooling ratio
Figure 1. Four concentrations of MS2 RNA were spiked at different positions in sample plates 1, 4 and 6 and used for the extraction protocol. qPCR results generated with the eluted RNA samples demonstrate that there is no significant difference in RNA recovery between the binding steps of MagSi-NA Pathogens MSP, and compared to the standard MagSi-NA Pathogens procedure without pooling.
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