rQ MagSi-NA Pathogens

Automation-ready total nucleic acid extraction for pathogen detection

rQ MagSi-NA Pathogens is an automation-ready-to-use kit for pathogen DNA and RNA extraction from a variety of sample materials like serum, plasma, oropharyngeal/nasopharyngeal swabs, or any other respiratory samples. It is based on the proven, market-leading MagSi-NA Pathogens kit. Components are delivered pre-filled in deepwell plates to be directly used in PurePrep 96, 32, 16 or KingFisher automated, nucleic acid purification systems.

Features

  • Conveniently pre-filled in plate formats
  • Minimal hands-on time; Less errors
  • More consistent and reproducible results
  • Consistently high yield of viral RNA
  • Very strong magnetic beads enable fast magnetic separation even from viscous sample lysates
  • Suitable for many enzymatic down-stream applications in particular RT-qPCR and sequencing
  • Run time on PurePrep systems is about 20 min
  • Ready-to-use on PurePrep 96, PurePrep 32/16 or KingFisher automated nucleic acid purification systems
  • Based on the proven, market-leading MagSi-NA Pathogens kit

Technical data

Figure 1.Ct values obtained by real time RT-PCR for BVDV/BDV after extraction with DirExtract Viral and competitor product. Ct values shown are mean values of 4 replicate extractions from ear punches from the same individual animal.

Figure 2.Ct values obtained from dilution series (10, 100, 1000x) of viral RNA extracted with DirExtract Viral from 2 different BVDV positive ear notches. For analysis 5 µL extracted RNA was combined with 10 µL real time RT-PCR master mix. Slopes of the regression lines demonstrate no significant PCR inhibition.

Resources

Resource

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Product leaflet - rQ MagSi-NA Pathogens

Manual - rQ MagSi-NA Pathogens

Magtivio catalog

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