rQ MagSi-NA Pathogens

Automation-ready total nucleic acid extraction for pathogen detection

rQ MagSi-NA Pathogens is an automation-ready-to-use kit for pathogen DNA and RNA extraction from a variety of sample materials like serum, plasma, oropharyngeal/nasopharyngeal swabs, or any other respiratory samples. It is based on the proven, market-leading MagSi-NA Pathogens kit. Components are delivered pre-filled in deepwell plates to be directly used in PurePrep 96, 32, 16 or KingFisher automated, nucleic acid purification systems.

Features

  • Conveniently pre-filled in plate formats
  • Minimal hands-on time; Less errors
  • More consistent and reproducible results
  • Consistently high yield of viral RNA
  • Very strong magnetic beads enable fast magnetic separation even from viscous sample lysates
  • Suitable for many enzymatic down-stream applications in particular RT-qPCR and sequencing
  • Run time on PurePrep systems is about 20 min
  • Ready-to-use on PurePrep 96, PurePrep 32/16 or KingFisher automated nucleic acid purification systems
  • Based on the proven, market-leading MagSi-NA Pathogens kit

Technical data

Figure 1. DNA concentrations obtained from 3 different donors with varying WBC counts (High, Medium, Low) with MagSi-DX Blood and a competitor kit (n=10).

Figure 2. Tapestation integrity assessment (Genomic DNA screentape) of DNA from whole blood stabilized with different anticoagulants from 2 donors. High DNA integrity numbers (DIN) were obtained, indicating high molecular weight DNA

Figure 3. PCR inhibition assessment of DNA after extraction from whole blood stabilized with different anticoagulants using MagSi-Dx Blood. DNA elutes were diluted in two 8-fold dilution steps and used as template in a real-time PCR targeting the ALB gene. Linear regression of the obtained Ct values vs dilution resulted in R2 ≥ 0.9995, indicating very good linearity.

Resources

Resource

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Product leaflet - rQ MagSi-NA Pathogens

Manual - rQ MagSi-NA Pathogens

Magtivio catalog

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