Pathogenic nucleic acids – DNA or RNA – are required to diagnose infections, monitor treatments and to track the spread of infections. These are all important tools in public health.
The MagSi-NA Pathogens kit allows for cost-effective extraction of pathogenic nucleic acids (DNA and RNA) from a variety of sample materials like serum, plasma, oropharyngeal swab, nasopharyngeal swab, or any other respiratory samples.
Use of nucleic acids
Purified total nucleic acids can be used for qPCR based or any other enzymatic pathogen detection method. The ready-to-use reagents and simple protocol are convenient in use and easy to automate. The included MagSi-PA VII magnetic beads are optimized for fast separation even from viscous sample lysates.
Short protocols, complete processing at room temperature possible
Consistently high yield of total nucleic acids
Very strong magnetic beads enable fast magnetic separation even from viscous sample lysates
Suitable for many enzymatic down-stream applications including qPCR, qRT-PCR isothermal amplification
Preparation time for 96 samples: <30 min
Figure 1.
SARS-CoV-2 detection. Variable amounts of SARS-CoV-2 positive saliva samples were diluted in SARS-CoV-2 negative saliva. RNA target sequences for SARS-CoV-2 (S- and N2-gene) were detected using a qRT-PCR assay. Homogeneous amplification of the endogenous control gene (IC, beta-Actin) was obtained in all samples, indicating absence of PCR inhibition.
ART.NO. | DESCRIPTION | AMOUNT |
|---|---|---|
MDKT00210096 | MagSi-NA Pathogens | 96 preps |
MDKT00210960 | MagSi-NA Pathogens | 10 x 96 preps |