Pathogenic nucleic acids – DNA or RNA – are required to diagnose infections, monitor treatments and to track the spread of infections. These are all important tools in public health.
The MagSi-NA Pathogens kit allows for cost-effective extraction of pathogenic nucleic acids (DNA and RNA) from a variety of sample materials like serum, plasma, oropharyngeal swab, nasopharyngeal swab, or any other respiratory samples.
Use of nucleic acids
Purified total nucleic acids can be used for qPCR based or any other enzymatic pathogen detection method. The ready-to-use reagents and simple protocol are convenient in use and easy to automate. The included MagSi-PA VII magnetic beads are optimized for fast separation even from viscous sample lysates.
Figure 1. Variable amounts of MS phage RNA were spiked to human serum samples. MS2 RNA was detected using a qRT-PCR assay. High recovery rates were obtained with reference to the spiked RNA (Input) and in comparison to a competitive kit (Product G).
Figure 2. Variable amounts of lambda DNA were spiked to human serum samples. Lambda DNA was detected using a qPCR assay. High recovery rates were obtained with reference to the spiked DNA (input) and in comparison to a competitive kit (Product G).
Figure 3. Variable amounts of SARS-CoV-2 positive saliva samples were diluted in SARS-CoV-2 negative saliva. RNA target sequences for SARS-CoV-2 (S- and N2-gene) were detected using a qRT-PCR assay. Homogeneous amplification of the endogenous control gene (IC, beta-Actin) was obtained in all samples, indicating absence of PCR inhibition.
RESOURCE | Language |
|---|---|
Product leaflet – MagSi-NA Pathogens | > EN |
Manual – MagSi-NA Pathogens | |
Technical Note – MagSi-NA Pathogens and KF-Flex | |
Technical Note – MagSi-NA Pathogens and KF-Flex one-step protocol | |
Technical Note – MagSi-NA Pathogens on Pureprep 96 | |
Technical Note – MagSi-NA Pathogens on Pureprep 96 (half volume) | |
Technical Note – MagSi-NA Pathogens on Pureprep 32 | |
Magtivio catalog |
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ART.NO.
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DESCRIPTION
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AMOUNT
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|---|---|---|
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MDKT00210096
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MagSi-NA Pathogens
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96 preps
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MDKT00210960
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MagSi-NA Pathogens
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10 x 96 preps
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