Blood is the most common and reliable source for genetic testing, particularly for genetic disorders. Nucleated white blood cells (WBCs) in whole blood contain high-quality DNA, making it ideal for large-scale genomic sequencing and mutation detection. Genetic testing can help detect inherited diseases (e.g., cystic fibrosis, sickle cell anemia), identify mutations in oncogenes or tumor suppressor genes, or determine how individuals respond to drugs based on their genetic profile.
MagSi-DX Blood is intended for manual and automated extraction of genomic DNA from fresh or frozen whole blood for subsequent in vitro diagnostics assays. It can be used with blood collection tubes containing EDTA, citrate or heparin as anticoagulant. Obtained genomic DNA from the MagSi-DX Blood kit has a high molecular weight, an important criteria for long-read technologies next to high quality, which makes this kit very suitable for long read sequencing.
Figure 1. DNA concentrations obtained from 3 different donors with varying WBC counts (High, Medium, Low) with MagSi-DX Blood and a competitor kit (n=10).
Figure 2. Tapestation integrity assessment (Genomic DNA screentape) of DNA from whole blood stabilized with different anticoagulants from 2 donors. High DNA integrity numbers (DIN) were obtained, indicating high molecular weight DNA
Figure 3. PCR inhibition assessment of DNA after extraction from whole blood stabilized with different anticoagulants using MagSi-Dx Blood. DNA elutes were diluted in two 8-fold dilution steps and used as template in a real-time PCR targeting the ALB gene. Linear regression of the obtained Ct values vs dilution resulted in R2 ≥ 0.9995, indicating very good linearity.
ART.NO. | DESCRIPTION | AMOUNT |
|---|---|---|
MDDX00020096 | MagSi-DX Blood | 96 preps |
MDDX00020960 | MagSi-DX Blood | 10 x 96 preps |