MagSi-DT Removal

Dye-Terminator removal from Sanger Sequencing

Magnetic bead-based MagSi-DT Removal offers an efficient solution for Dye-Terminator removal from Sanger (BigDye®) sequencing reactions.

Post-cycle sequencing reaction contaminants that interfere with sequencing analysis (in particular unincorporated dyes) are removed by a rapid clean-up method without centrifugation or filtration.

The kit can be used in high-throughput processes with 96 and 384 well plates on automated liquid handling platforms. It is optimized for use on Biomek® Laboratory Automation Workstations and Hamilton® Microlab® STARTM Line.

Features

High quality results

  • Efficient removal of unincorporated Dye-Terminators and salts
  • High signal intensities and long Phred 20 read lengths
  • High pass rates
  • Consistent performance

 

Flexible and cost-saving

  • Straightforward protocol with bind-wash-elute procedure
  • No need for centrifugation or filtration
  • Competitive pricing
  • Save reagent costs by using only 0.5 μL BigDye®
  • Clean-up directly in reaction plates

 

Easy to automate

  • Identical protocol as competitor magnetic beads Agencourt CleanSEQ®
  • Optimized for use on Biomek® Laboratory Automation Workstations and Hamilton® Microlab STARline
  • Compatible with many other different automated liquid handling systems (e.g. PerkinElmer®, Caliper Life Sciences®, etc.)

Technical data

Figure 1. Sequence electropherogram (trace file)

Resources

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