FFPE stands for “Formalin-Fixed Paraffin-Embedded,” and it refers to a common method of preserving and storing biological tissue samples for histological analysis and molecular studies. FFPE tissue is widely used in pathology, research, and clinical diagnostics.
The MagSi-DNA FFPE kit is intended for manual and automated isolation of genomic DNA from mammalian FFPE tissue or cell samples. Processing time for the preparation of 96 samples is about 40 minutes plus an additional paraffin removal step and lysis incubation.
Safety
The kit does not require phenol/chloroform extraction or alcohol precipitation and eliminates the need for repeated centrifugation, vacuum filtration or column separation. It allows safe handling of potentially infectious samples, and is designed to avoid sample-to-sample cross-contaminations. The obtained DNA can be used directly for downstream applications such as PCR, NGS, or any kind of enzymatic reaction.
Figure 1. DNA concentrations (NanoDrop) obtained from different FFPE samples (liver, lung, cells) after extraction vs competitor.
Figure 2. DNA concentrations (NanoDrop) obtained from 1 to 4 scrolls (20 µm) of FFPE embedded cells. Corresponding DNA purity ratios A260/A280 range from 1.74 to 1.89. Sample deparaffinization was achieved using the melting procedure by heat incubation without xylene, mineral oil or other organic solvents.
Figure 3. Ct values obtained by qPCR (beta-actin) on purified lung, liver and cell samples versus competitor. Purified samples were used undiluted in the qPCR reaction. No PCR inhibition was observed.
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ART.NO.
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DESCRIPTION
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AMOUNT
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MDKT00240096
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MagSi-DNA FFPE
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96 preps
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MDKT00240960
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MagSi-DNA FFPE
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10 x 96 preps
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