From disease diagnostics and genetic screening to pedigree verification and wildlife conservation, the multifaceted applications of Animal DNA have ushered in a new era of precision and reliability in assessing animals’ health, traits, and relationships.
The MagSi-DNA Animal kit allows for fast and cost-effective extraction of genomic DNA from various animals and samples like blood, semen, hairs, saliva/swabs or lysed tissue.
Universal
This universal DNA purification kit is optimized to extract DNA from sample materials with the highest purity and delivers DNA which is suitable for genotyping assays or other PCR based analyses.
Validated chemistry
The extraction chemistry has been validated on many different animal species, e.g. horse, swine, dog, cattle, fish, shrimp, insects and molluscs and can be customized to meet any specific requirements of yields, purity, working volumes.
General features
Quality
Flexibility
Easy to Automate
Figure 1. Nucleic acid concentration obtained from DNA extractions from whole blood of different animal species (n=3). Chicken and turkey blood was lysed for 1 hour, mammalian blood was lysed for 10 min.
Figure 2. DNA purity obtained after DNA extractions from whole blood of different animal species. All purity ratios indicate highly pure DNA (A260/A280 > 1.7).
Figure 3. Ct values obtained from qPCR after extraction from whole blood of different animal species (n=3). For Chicken and turkey 1 μL eluate, for all others 4 μL eluate, where used as PCR input.
Figure 4. DNA concentrations of different fish species obtained with the QubitTM dsDNA BR Assay Kit. The different input amounts (10, 20 and 40 mg) resulted in a DNA yield ranging from 16 to 225 ng/μL. The data presented are mean values (n= 8, ±1 SD).
Figure 5. DNA purity ratios of different fish species obtained by UV-VIS with the NanoDropTM One. The data presented are mean values of DNA extractions from 20 mg sample (n= 8, ±1 SD).
Figure 6. Ct values obtained from DNA extracted from trout and cod fin by PCR using primers targeting the mitochondrial cytochrome oxidase subunit I gene (cox1). DNA samples were 1:10 diluted before PCR. Ct values between 18 and 22 are reported by the AriaMx Real-Time PCR system (10, 20, 40 mg). The data presented are mean values (n= 8, ±1 SD).
Figure 7. DNA concentrations obtained with the QubitTM dsDNA BR Assay Kit. The different shrimp parts resulted in a DNA yield ranging from 21 to 115 ng/μL. The data are presented as mean (n=8, ±1 SD).
Figure 9. Ct values for different shrimp parts. Ct values range from 17 to 27. The data are presented as mean (n=8, ±1 SD).
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Resource
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Language
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Product leaflet - MagSi-DNA Animal
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Manual - MagSi-DNA Animal
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Application Note - Data from various animal samples
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> EN
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Application Note - DNA extraction from whole blood for genetic testing in livestock management
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> EN
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Application Note - DNA extraction from fish fins for genetic testing by aquaculture laboratories
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> EN
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Application Note - DNA extraction from shrimp samples for genomics-assisted breeding
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> EN
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Application Note - DNA extraction from molluscs for marker-assisted breeding
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> EN
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Application Note - DNA extraction from honey bees for parasite resistance analysis
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> EN
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Application Note - DNA extraction from edible insects
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> EN
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Technical Note – MagSi-DNA Animal and KingFisher-Flex
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> EN
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Magtivio catalog
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ART. NO.
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Description
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Amount
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MDKT00150096
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