Cattle ear notch testing for Bovine Viral Diarrhea Virus (BVDV) analysis holds significant relevance in the field of animal health and farm management. By examining ear notches from cattle, this testing method provides essential insights into the presence and prevalence of BVDV infections within herds.
As BVDV can lead to reproductive losses, reduced productivity, and immune suppression in cattle, early detection and monitoring are crucial for preventing its spread and minimizing its economic impact. Ear notch testing allows veterinarians, farmers, and researchers to identify infected animals, implement effective control strategies, and make informed decisions about herd health.
Figure 1.Ct values obtained by real time RT-PCR for BVDV/BDV after extraction with DirExtract Viral and competitor product. Ct values shown are mean values of 4 replicate extractions from ear punches from the same individual animal.
Figure 2.Ct values obtained from dilution series (10, 100, 1000x) of viral RNA extracted with DirExtract Viral from 2 different BVDV positive ear notches. For analysis 5 µL extracted RNA was combined with 10 µL real time RT-PCR master mix. Slopes of the regression lines demonstrate no significant PCR inhibition.
ART.NO. | DESCRIPTION | AMOUNT |
|---|---|---|
MDRE00180010 | DirExtract Viral | 10 mL / 100 preps |
MDRE00180100 | DirExtract Viral | 100 mL / 1000 preps |