MagSi-NGSPREP Plus

Size selection and clean-up in NGS library preparation

Magnetic bead-based MagSi-NGSPREP Plus offers an efficient solution for both size selection and clean-up of the successive enzymatic reactions in library preparation for NGS applications. The simple and flexible protocol can be adjusted to your specific library preparation kit and NGS instrument. MagSi-NGSPREP Plus can be used manually but is also easy to automate for high-throughput processing. In conclusion: a simple and quick way to consistent sequencing results.

The MagSi-NGSPREP Plus kit is optimized for use on Biomek® Laboratory Automation Workstations and Hamilton® MicroLab STARline. MagSi-NGSPREP Plus allows either non-selective binding, or size-targeted binding of double-stranded DNA fragments ranging from 80 – 1000 bp with specific reagent volume to sample volume ratio’s. By increasing the volume of MagSi-NGSPREP Plus, the efficiency of binding smaller fragments increases. This enables the user to selectively keep or discard undesired fragment sizes.

MagSi-NGSPREP Plus’ flexible protocols are easy to automate for high-throughput screening.

High quality results

  • High recovery of PCR products
  • Excellent removal of enzymes, primers, oligonucleotides, polymerases and other contaminants
  • Fragment size selection adjustable between 100 and 1000 base pairs
  • Guarantees consistent sequencing results


Simplify your routine

  • One product for all clean-up and size selection steps in the library preparation work-flow
  • Simple bind-wash-elute procedure with short process time
  • Protocol easily adjustable for clean-up or size selection using specific reagent-to-sample ratios
  • Manual and automate use
  • Compatible with standard protocols of common library preparation kits
ART.NO.
PRODUCT
VOLUME
MDKT00010005
MagSi-NGSPREP Plus
5 mL
MDKT00010075
MagSi-NGSPREP Plus
75 mL
MDKT00010500
MagSi-NGSPREP Plus
500 mL

DNA size distribution curves after left-sided size selection showing a direct comparison between MagSi-NGSPREP Plus and AMPure® XP. Four different bead-to-sample ratios were used.

 

 

Agarose gel showing 6 independent size selections from a DNA ladder, using ratios of 0.65x (left) and 1.0x (right)

TECHNICAL DATA | MagSi-NGSPREP Plus

Fig 1 and 2: Left Side Size Selection fraction retained by beads with different reagent-to-sample volume ratios on TapeStation ScreenTape.

PRODUCT RESOURCES | MagSi-NGSPREP Plus

Catalog

All our magnetic beads, kits and separators in one overview.

Leaflets

Commercial leaflets of our magnetic separation products.

Manuals

Manuals on how to use our magnetic separation solutions.