Size selection and clean-up in NGS library preparation
Magnetic bead-based MagSi-NGSPREP Plus offers an efficient solution for both size selection and clean-up of the successive enzymatic reactions in library preparation for NGS applications. The simple and flexible protocol can be adjusted to your specific library preparation kit and NGS instrument. MagSi-NGSPREP Plus can be used manually but is also easy to automate for high-throughput processing. In conclusion: a simple and quick way to consistent sequencing results.
The MagSi-NGSPREP Plus kit is optimized for use on Biomek® Laboratory Automation Workstations and Hamilton® MicroLab STARline. MagSi-NGSPREP Plus allows either non-selective binding, or size-targeted binding of double-stranded DNA fragments ranging from 80 – 1000 bp with specific reagent volume to sample volume ratio’s. By increasing the volume of MagSi-NGSPREP Plus, the efficiency of binding smaller fragments increases. This enables the user to selectively keep or discard undesired fragment sizes.
MagSi-NGSPREP Plus’ flexible protocols are easy to automate for high-throughput screening.
ART.NO.
|
PRODUCT
|
VOLUME
|
---|---|---|
MDKT00010005
|
MagSi-NGSPREP Plus
|
5 mL
|
MDKT00010075
|
MagSi-NGSPREP Plus
|
75 mL
|
MDKT00010500
|
MagSi-NGSPREP Plus
|
500 mL
|
DNA size distribution curves after left-sided size selection showing a direct comparison between MagSi-NGSPREP Plus and AMPure® XP. Four different bead-to-sample ratios were used.
Agarose gel showing 6 independent size selections from a DNA ladder, using ratios of 0.65x (left) and 1.0x (right)
Figures 1 and 2.
Left side size selection fraction retained by beads with different reagent-to-sample volume ratios on TapeStation ScreenTape.
All our magnetic beads, kits and separators in one overview.
Commercial leaflets of our magnetic separation products.
Manuals on how to use our magnetic separation solutions.
An overview of magtivio (and its predecessor MagnaMedics) product’s scientific publications.