MagSi-NGSPREP Plus

Size selection and clean-up in NGS library preparation

Magnetic bead-based MagSi-NGSPREP Plus offers an efficient solution for both size selection and clean-up of the successive enzymatic reactions in library preparation for NGS applications. The simple and flexible protocol can be adjusted to your specific library preparation kit and NGS instrument. MagSi-NGSPREP Plus can be used manually but is also easy to automate for high-throughput processing. In conclusion: a simple and quick way to consistent sequencing results.

The MagSi-NGSPREP Plus kit is optimized for use on Biomek® Laboratory Automation Workstations and Hamilton® MicroLab STARline. MagSi-NGSPREP Plus allows either non-selective binding, or size-targeted binding of double-stranded DNA fragments ranging from 80 – 1000 bp with specific reagent volume to sample volume ratio’s. By increasing the volume of MagSi-NGSPREP Plus, the efficiency of binding smaller fragments increases. This enables the user to selectively keep or discard undesired fragment sizes.

MagSi-NGSPREP Plus’ flexible protocols are easy to automate for high-throughput screening.

High quality results

  • High recovery of PCR products
  • Excellent removal of enzymes, primers, oligonucleotides, polymerases and other contaminants
  • Fragment size selection adjustable between 100 and 1000 base pairs
  • Guarantees consistent sequencing results


Simplify your routine

  • One product for all clean-up and size selection steps in the library preparation work-flow
  • Simple bind-wash-elute procedure with short process time
  • Protocol easily adjustable for clean-up or size selection using specific reagent-to-sample ratios
  • Manual and automate use
  • Compatible with standard protocols of common library preparation kits
ART.NO.
PRODUCT
VOLUME
MDKT00010005
MagSi-NGSPREP Plus
5 mL
MDKT00010075
MagSi-NGSPREP Plus
75 mL
MDKT00010500
MagSi-NGSPREP Plus
500 mL

DNA size distribution curves after left-sided size selection showing a direct comparison between MagSi-NGSPREP Plus and AMPure® XP. Four different bead-to-sample ratios were used.

 

 

Agarose gel showing 6 independent size selections from a DNA ladder, using ratios of 0.65x (left) and 1.0x (right)

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