MagSiMUS - LCMS Sample Prep
Remove the haystack, to find the needle
The MagSiMUS Principle
More and more (U)HPLC and Mass Spectrometry technologies replace the less sensitive and often more unspecific immunoassays for the detection and analysis of increasingly complex target analytes in clinical diagnostics and screening laboratories. To reduce matrix effects, a good sample preparation method upfront of these analyses is often required to exclude interfering compounds – especially proteins. All commonly used methods – Protein Precipitation (PP), Solid Phase Extraction (SPE) and Liquid Liquid Extraction (LLE) – have specific disadvantanges: difficult to automate, time-consuming and/or economically unfavorable.
MagSiMUS magnetic bead-based biological sample preparation kits are intended for protein depletion and removal of other interfering compounds from biological samples like whole blood, serum, plasma or urine. They work according to the unique and innovative negative selection technology, which removes the contaminants and leaves the target molecules in the supernatant.
For LC-MS, an internal standard, typically dissolved in dilution reagent, is added to a clinical sample. This ensures that the analyte itself and the internal standard undergo one and the same cleanup route. The MagSiMUS magnetic bead (particle) mix is then added and suspended homogeneously into the clinical sample. Proteins and larger peptides are then precipitated towards the beads’ surface by addition of a protein precipitating reagent. The magnetic bead/protein precipitate is subsequently attracted by a magnetic field with a suitable magnetic separator, leaving the target analyte in suspension. The supernatant is now clean and can be directly injected into the readout system (UHPLC, LC-MS/MS).
Remove the Haystack…To Find the Needle
MagSiMUS technology uses flexible protocols, does not require centrifugation and is easy to automate for medium and high-throughput processing.
MagSiMUS methods are designed and optimized for the sample cleanup prior to the LC-MS/MS or (U)HPLC analysis of specific analytes. For more information on which MagSiMUS method to use for what analytes, contact our technical support department.
MagSiMUS features
- Reduced matrix effects in (U)HPLC and Mass Spectrometry
- Quick and complete sample preparation protocols, with short run times
- Easy to automate for medium and high throughputs
- Works with low sample volumes (down to 25 – 50 µL) so there is always cost-saving on reference materials
- No need for centrifugation or the application of positive or negative (air) pressure
- Different MagSiMUS methods available in kit formats covering multiple analytes.
Sample Preparation for Therapeutic Drug Monitoring (TDM) by (U)HPLC or LC-MS
MagSiMUS-TDMPREP
MagSiMUS-TDMPREP kits are designed for the cleanup of biological sample materials prior to LC-MS/MS or (U)HPLC analysis of e.g. immunosuppressants, antiepileptics, anticoagulants, neuroleptics, antimycotics, psychoactive drugs, antiarrhythmics, antibiotics and many other therapeutic drugs or substances.
MagSiMUS-TDMPREP kits are available in two different magnetic bead type versions: Type I and Type II. Each magnetic bead mix type is optimized for the recovery of the analytes / panels of interest. The sample preparation method is the same for both bead mix types. Organic Precipitation Reagents (OPR I or OPR VI) for the selected method should be ordered separately, as well as Lysis Buffer for the whole blood protocol.
For more information and selection of the right magnetic bead type, contact our technical support department.
Art.No.
product
volume
MD03026
MagSiMUS-TDMPREP Type I (contains Type I Particle Mix, ISDR A and ISDR B)
500 preps
MD03226
MagSiMUS-TDMPREP Type II (contains Type II Particle Mix, ISDR A and ISDR B)
500 preps
Materials that can be ordered separately
MD71130
Organic Precipitation Reagent I (OPR I); for acetonitrile-based precipitation
100 mL
MD71335
Organic Precipitation Reagent VI (OPR VI); for methanol-based precipitation
100 mL
MD71630
Lysis Buffer for whole blood
100 mL
MDRE00110100
Internal Standard Dilution Reagent A (ISDR A) – for use with OPR I
100 mL
MD71530
Internal Standard Dilution Reagent B (ISDR B) – for use with OPR VI
100 mL
Sample Preparation for Toxicology and Drugs of Abuse (DoA) Screening
MagSiMUS-TOXPREP
MagSiMUS-TOXPREP kits are aimed at the cleanup of biological sample materials prior to LC-MS/MS or (U)HPLC analysis of e.g. benzodiazepines, metabolites, vitamins, drugs of abuse (amphetamines, cannabinoids, barbiturates, morphines), antidepressants and many other abusive drugs or substances.
MagSiMUS-TOXPREP kits are available in two magnetic bead type versions: Type I and Type II. Each of these 2 different magnetic bead mix types is optimized for the recovery of the analyte of interest. The sample preparation method is the same for both bead mix types. Organic Precipitation Reagents (OPR I or OPR VI) for the selected method should be ordered separately, as well as Lysis Buffer for the whole blood protocol.
For more information and selection of the right magnetic bead type, contact our technical support department.
Art.No.
product
volume
MD03028
MagSiMUS-TOXPREP Type I (contains Type I Particle Mix, ISDR A and ISDR B)
500 preps
MD03128
MagSiMUS-TOXPREP Type II (contains Type II Particle Mix, ISDR A and ISDR B)
500 preps
Materials that can be ordered separately
MD71130
Organic Precipitation Reagent I (OPR I); for acetonitrile-based precipitation
100 mL
MD71335
Organic Precipitation Reagent VI (OPR VI); for methanol-based precipitation
100 mL
MD71630
Lysis Buffer for whole blood
100 mL
MD71730
Urine Stabilization Buffer; for stabilization of pH in urine samples
10 mL
MDRE00110100
Internal Standard Dilution Reagent A (ISDR A) – for use with OPR I
100 mL
MD71530
Internal Standard Dilution Reagent B (ISDR B) – for use with OPR VI
100 mL
Accessory products for MagSiMUS Sample Preparation
Accessory reagents/buffers for the preparation and dilution of internal standards, precipitation and for sample lysis and stabilization steps in the MagSiMUS protocols. These reagents can be components of MagSiMUS kits, or should be ordered separately with MagSiMUS kits.
Art.No.
product
volume
MD71130
Organic Precipitation Reagent I (OPR I); for acetonitrile-based precipitation
100 mL
MD71335
Organic Precipitation Reagent VI (OPR VI); for methanol-based precipitation
100 mL
MDRE00110100
Internal Standard Dilution Reagent A (ISDR A) – for use with OPR I
100 mL
MD71530
Internal Standard Dilution Reagent B (ISDR B) – for use with OPR VI
100 mL
MD71630
Lysis Buffer for whole blood
100 mL
MD71730
Urine Stabilization Buffer; for stabilization of pH in urine samples
10 mL
product resources | magsimus
Leaflets
Commercial leaflets of our magnetic separation products.
Manuals
Manuals on how to use our magnetic separation solutions.
Publications
An overview of magtivio (and its predecessor MagnaMedics) product’s scientific publications.