Reduction of protein or peptide complexicity
MagSi-WCX (Weak Cation eXchange) magnetic beads have the typical ion exchange properties well known from classical chromatography. Protein and peptides are charged at the surface and will be adsorbed to magnetic beads under low salt condition. Under high salt conditions or by pH shift the target proteins/peptides are eluted (ion exchanged). Due to the different binding/elution mechanisms the MagSi-WCX beads are a powerful alternative to the MagSi proteomics beads.
Magnetic silica particles with weak cation exchange surface (WCX). MagSi-WCX is ideal for the reduction of protein or peptide complexity. Applications include sample preparation and pre-fractionation prior to mass spectrometry or SDS-PAGE analysis, biomarker analysis and serum /plasma profiling.
MagSi-WAX (Weak Anion eXchange) magnetic beads are the “counter” beads to the MagSi WCX beads. Binding, washing and elution are also triggered by the protein/peptide total net charge. The MagSi-WAX beads are ideally suited when more acidic proteins/peptides are expected in the sample. However, our protocols given in the corresponding product sheet cover buffer systems for acidic, neutral and basic conditions.
MagSi-WAX beads are magnetic silica beads with a high magnetic content optimized for protein and peptide separation. Since the MagSi-WCX and MagSi-WAX act as counter-beads, MagnaMedics recommends to test both types of beads, to see which suits best in your individual application.
Magnetic silica particles with weak anion exchange surface (WAX). Similar to MagSi-WCX, MagSi-WAX is ideal for the reduction of protein or peptide complexity. Applications include sample preparation and pre-fractionation prior to mass spectrometry or SDS-PAGE analysis, biomarker analysis and serum /plasma profiling.