Fast and Cost-effective Extraction of Genomic DNA from blood

MagSi-gDNA blood kit allows fast and cost-effective extraction of genomic DNA from a wide range of sample volumes. The paramagnetic bead-based kit can be used on whole blood samples drawn in EDTA-, citrate- or heparin tubes. Due to the ready to use reagents and simple protocol the kit is easy to use and easy to automate. As a linear volume to volume ratio is used between whole blood sample and reagents, it is possible to use the kit in any situation where genomic DNA out of blood is needed.

Features

  • Suitable for whole blood, drawn in Citrate-, EDTA- or Heparin tubes
  • Reproducible, high yield of DNA – up to 8 μg from 200 μL fresh or frozen whole blood
  • Excellent purity, A260/280 = 1.7-2.0
  • Suitable for many genomic applications including PCR, DNA sequencing and Southern blotting
  • Simple bind-wash-elute procedure
  • Protocol easily adjustable for different sample volumes
  • Manual and automated use
  • Safe sample handling with minimal user input
  • Also suitable for isolation of DNA from saliva, serum/plasma and cultured cells

Easy to automate

  • Compatible with many different automated liquid handling systems (e.g KingFisherTM, PrimaRWS®, PIPETMAX® and JANUS®)
  • Short and easy protocols
  • Suitable for small, medium and high-throughput automation
  • Variable volume sample input possible

 

Product Starting Material Average Yield Preparation Time
MagSi gDNA blood kit Up to 200 μl whole blood 3 – 15 μg, depending on the blood sample (storage and source) A260/A280: 1.7-2.0 Approx. 20-25 min. after lysis

 

DNA isolation from human whole blood

Genomic DNA gel
Figure 1. Gel electrophoresis of genomic DNA extracted from 50-200 μL fresh whole blood. M: 1 kb Extension ladder (Life Technologies)
UV-VIS Absorbance spectrum
Figure 2. UV-VIS Absorbance spectrum of DNA isolated from 100, 150 and 200 μL fresh whole blood

 

 

 

 

 

 

 

This magnetic bead-based kit is a fast and economic purification system for extraction of genomic DNA from mammalian samples. The MagSi-gDNA blood kit includes magnetic beads, buffers and Proteinase K, and is easy to automate for high-throughput processing.

 

Art.No. Product Volume
MD60001 MagSi-gDNA blood kit 96 96 preps
MD61001 MagSi-gDNA blood kit 10 x 96 10 x 96 preps
MD62001 MagSi-gDNA blood kit 10 x 960 (bulk) 10 x 960 preps